| Type of Material: | Thesis |
| Title: | Cloning and characterisation of cdna encoding legume seed defensin and its activity against bruchids |
| Researcher: | Dayma, Jyotsna |
| Guide: | Acharjee, Sumita |
| Department: | Department of Agricultural Biotechnology |
| Publisher: | Assam Agricultural University, Johrat |
| Place: | Johrat |
| Year: | 2023 |
| Language: | English |
| Subject: | Agricultural Sciences | Agriculture Multidisciplinary | Life Sciences | Life Sciences | Life Science |
| Dissertation/Thesis Note: | PhD; Department of Agricultural Biotechnology, Assam Agricultural University, Johrat, Johrat; 2023 |
| Fulltext: | Shodhganga |
| 003 | IN-AhILN | |
| 005 | 2026-09-18 15:31:22 | |
| 008 | __ | 260918t2023||||ii#||||g|m||||||||||eng|| |
| 035 | __ | |a(IN-AhILN)th_463304 |
| 040 | __ | |dIN-AhILN|aASAM_785013 |
| 041 | __ | |aeng |
| 100 | __ | |eResearcher|aDayma, Jyotsna |
| 110 | __ | |aDepartment of Agricultural Biotechnology|0U-0048|dJohrat|bAssam Agricultural University, Johrat|ein |
| 245 | __ | |aCloning and characterisation of cdna encoding legume seed defensin and its activity against bruchids |
| 260 | __ | |aJohrat|c2023|bAssam Agricultural University, Johrat |
| 300 | __ | |dDVD |
| 502 | __ | |d2023|bPhD|cDepartment of Agricultural Biotechnology, Assam Agricultural University, Johrat, Johrat |
| 518 | __ | |d2023|oDate of Award |
| 518 | __ | |d2018|oDate of Registration |
| 518 | __ | |oDate of Completion|d2023 |
| 520 | __ | |aDefensins are small cysteine-rich peptides that play a crucial role in a plant s innate immune system and are known for their well-established antimicrobial activity. However, their potential insecticidal properties have received limited exploration. Chen and his coworker have identified a defensin protein from mung bean (Vigna radiata) having insecticidal activity against bruchids. Our previous research confirmed the upregulation of a defensin gene in black gram due to the bruchid infestation. In the present study, sequencing of the full-length complementary DNA of the defensin gene from various legumes was performed. We found two amino acid sequence variants, PEP1 and PEP2 with 90 to 97% homology with the previously reported mung bean defensin protein, respectively. The PEP1 variant was found in black gram, pea, cowpea, and common bean, while PEP2 was found in mung bean, chickpea, and pigeon pea. The amino acid sequence alignment showed there are two amino acid substitutions in the signal peptide and thre |
| 650 | __ | |aLife Sciences|2UGC |
| 650 | __ | |aLife Science|2AIU |
| 653 | __ | |aAgricultural Sciences |
| 653 | __ | |aAgriculture Multidisciplinary |
| 653 | __ | |aLife Sciences |
| 700 | __ | |aAcharjee, Sumita|eGuide |
| 856 | __ | |uhttp://shodhganga.inflibnet.ac.in/handle/10603/583715|yShodhganga |
| 905 | __ | |afromsg |
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