Title : Diversity of bacteriophages infecting ralstonia solanacearum and their application as bio control agents

Type of Material: Thesis
Title: Diversity of bacteriophages infecting ralstonia solanacearum and their application as bio control agents
Researcher: Barua, Parinda
Guide: Nath, P. D.
Department: Plant Pathology
Publisher: Assam Agricultural University, Johrat
Place: Johrat
Year: 2021
Language: English
Subject: Plant Pathology
Biotechnology
Life Science
Dissertation/Thesis Note: PhD; Plant Pathology, Assam Agricultural University, Johrat, Johrat; 2021
Fulltext: Shodhganga

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035__|a(IN-AhILN)th_462938
040__|dIN-AhILN|aASAM_785013
041__|aeng
100__|eResearcher|aBarua, Parinda
110__|aPlant Pathology|0U-0048|dJohrat|bAssam Agricultural University, Johrat|ein
245__|aDiversity of bacteriophages infecting ralstonia solanacearum and their application as bio control agents
260__|aJohrat|c2021|bAssam Agricultural University, Johrat
300__|dDVD
502__|d2021|bPhD|cPlant Pathology, Assam Agricultural University, Johrat, Johrat
518__|d2021|oDate of Award
518__|oDate of Registration|d2017
518__|oDate of Completion|d2021
520__|aBacterial wilt caused by Ralstonia solanacearum is one of the most destructive plant diseases of the world that is very difficult to manage. Sustainable management of R. solanacearum using bacteriophages is one of the most promising yet unexplored areas of biological control in India. In the present study, 50 R. solanacearum isolates were collected from various bacterial wilt infected fields in 5 districts of Upper Brahmaputra Valley Zone (UBVZ) of Assam namely Golaghat, Jorhat and Sivsagar, Dibrugarh and Tinsukia from several crops like bhut jolokia, chilli, capsicum, tomato, brinjal, wild brinjal, potato and ginger. This was followed by isolation of seven lytic phages of R. solanacearum from soil from bhut jolokia fields of Jorhat, Assam. After six rounds of purification of the phages, the plaque morphology, phage titre and the multiplicity of infection (MOI) was determined where it was observed that phage RSP1 had the biggest plaque size (3.28 ± 0.40 nm) and phage RSP4 had the highest titre (1.2E+09 PFU
650__|aBiotechnology|2UGC
650__|aLife Science|2AIU
653__|aPlant Pathology
700__|aNath, P. D.|eGuide
856__|uhttp://shodhganga.inflibnet.ac.in/handle/10603/487313|yShodhganga
905__|afromsg

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