Title : Morphological and functional characterization of boar spermatozoa on incubation in capacitating media and preservation

Type of Material: Thesis
Title: Morphological and functional characterization of boar spermatozoa on incubation in capacitating media and preservation
Researcher: Das, Arunima
Guide: Barua, P M
Department: Animal Reproduction, Gynaecology and Obstetrics
Publisher: Assam Agricultural University, Johrat
Place: Johrat
Year: 2022
Language: English
Subject: Animal Science
Biotechnology
Life Science
Dissertation/Thesis Note: PhD; Animal Reproduction, Gynaecology and Obstetrics, Assam Agricultural University, Johrat, Johrat; 2022
Fulltext: Shodhganga

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035__|a(IN-AhILN)th_462935
040__|dIN-AhILN|aASAM_785013
041__|aeng
100__|eResearcher|aDas, Arunima
110__|aAnimal Reproduction, Gynaecology and Obstetrics|0U-0048|dJohrat|bAssam Agricultural University, Johrat|ein
245__|aMorphological and functional characterization of boar spermatozoa on incubation in capacitating media and preservation
260__|aJohrat|c2022|bAssam Agricultural University, Johrat
502__|bPhD|d2022|cAnimal Reproduction, Gynaecology and Obstetrics, Assam Agricultural University, Johrat, Johrat
518__|oDate of Award|d2022
518__|oDate of Registration|d2007
518__|oDate of Completion|d2022
520__|aA total of 24 ejaculates comprising 6 ejaculates from each of four HD-K75 boars of 10-12 months age maintained at ICAR - All India Coordinated Research Project (AICRP) on Pig C.V.Sc, A.A.U., Khanapara, Guwahati are being selected for the present study. The semen was collected by simple fist method twice weekly to study the morphological and functional characterization of in-vitro capacitated and preserved boar spermatozoa. After initial evaluation (volume, concentration and initial motility), the fresh semen was split into three parts. One part of the semen was used for fresh semen evaluation, second for capacitation and the other for preservation. For capacitation, the semen was incubated in TALP and m-KRB media at 37oC for 5 hours. For preservation semen was extended (1:4) in BTS and GEPS extenders and held at 22ºC for 4 hours. The extended semen was then preserved at 15oC in BOD incubator upto 120 hours. The overall mean of strained volume of semen, initial motility, hyperactivated spermatozoa, sperm co
650__|aBiotechnology|2UGC
650__|aLife Science|2AIU
653__|aAnimal Science
700__|aBarua, P M|eGuide
856__|uhttp://shodhganga.inflibnet.ac.in/handle/10603/476666|yShodhganga
905__|afromsg

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