| Type of Material: | Thesis |
| Title: | Development of RNAi construct and optimization of in vitro regeneration and transformation protocol against banana bunchy top virus |
| Researcher: | Kaushik, Dipshikha |
| Guide: | Nath, P. D. |
| Department: | Plant Pathology |
| Publisher: | Assam Agricultural University, Johrat |
| Place: | Johrat |
| Year: | 2021 |
| Language: | English |
| Subject: | Plant Pathology | Agricultural Sciences | Life Science |
| Dissertation/Thesis Note: | PhD; Plant Pathology, Assam Agricultural University, Johrat, Johrat; 2021 |
| Fulltext: | Shodhganga |
| 003 | IN-AhILN | |
| 005 | 2026-09-10 17:22:40 | |
| 008 | __ | 260910t2021||||ii#||||g|m||||||||||eng|| |
| 035 | __ | |a(IN-AhILN)th_462929 |
| 040 | __ | |dIN-AhILN|aASAM_785013 |
| 041 | __ | |aeng |
| 100 | __ | |eResearcher|aKaushik, Dipshikha |
| 110 | __ | |aPlant Pathology|0U-0048|dJohrat|bAssam Agricultural University, Johrat|ein |
| 245 | __ | |aDevelopment of RNAi construct and optimization of in vitro regeneration and transformation protocol against banana bunchy top virus |
| 260 | __ | |aJohrat|c2021|bAssam Agricultural University, Johrat |
| 300 | __ | |dDVD |
| 502 | __ | |d2021|bPhD|cPlant Pathology, Assam Agricultural University, Johrat, Johrat |
| 518 | __ | |d2021|oDate of Award |
| 518 | __ | |oDate of Registration|d2015 |
| 518 | __ | |oDate of Completion|d2021 |
| 520 | __ | |aBanana bunchy top disease (BBTD) is one of the most destructive viral diseases responsible for huge destruction in the crop yield, caused by the banana bunchy top virus (BBTV). Currently, there are no strategies available to completely protect bananas against the BBTV. Therefore, in this study, an attempt was made to develop an RNAi construct for bunchy top disease management in bananas. Here, Nuclear shuttle protein (NSP) gene encoded by BBTV DNA-6 was targeted. The Coat protein gene (CP) encoded by BBTV DNA-3 and Nuclear shuttle protein(NSP) gene encoded by BBTV DNA-6 were initially cloned in pGEMT-easy vector. The clones were confirmed by colony PCR, restriction digestion, and sequencing. Sequence results were 96.69% and 94.18% similar to BBTV accessions KX592198 and KX592200 for BBTV-DNA-3 and BBTV-DNA-6 sequences, respectively. Also, NSP gene fragment (500bp) was successfully cloned in sense orientation in an RNAi vector pHANNIBAL. For the process of callus induction, 1-10 weeks old immature male flowe |
| 650 | __ | |aAgricultural Sciences|2UGC |
| 650 | __ | |aLife Science|2AIU |
| 653 | __ | |aPlant Pathology |
| 700 | __ | |aNath, P. D.|eGuide |
| 856 | __ | |uhttp://shodhganga.inflibnet.ac.in/handle/10603/474174|yShodhganga |
| 905 | __ | |afromsg |
User Feedback Comes Under This section.