| Type of Material: | Thesis |
| Title: | Production and characterization of recombinant beta toxin of clostridium perfringens |
| Researcher: | Bharali, Arpita |
| Guide: | Sharma, R K |
| Department: | Animal Biotechnology |
| Publisher: | Assam Agricultural University, Johrat |
| Place: | Johrat |
| Year: | 2022 |
| Language: | English |
| Subject: | Plant and Animal Science | Biotechnology | Life Science |
| Dissertation/Thesis Note: | PhD; Animal Biotechnology, Assam Agricultural University, Johrat, Johrat; 2022 |
| Fulltext: | Shodhganga |
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| 008 | __ | 260910t2022||||ii#||||g|m||||||||||eng|| |
| 035 | __ | |a(IN-AhILN)th_462905 |
| 040 | __ | |dIN-AhILN|aASAM_785013 |
| 041 | __ | |aeng |
| 100 | __ | |eResearcher|aBharali, Arpita |
| 110 | __ | |aAnimal Biotechnology|0U-0048|dJohrat|bAssam Agricultural University, Johrat|ein |
| 245 | __ | |aProduction and characterization of recombinant beta toxin of clostridium perfringens |
| 260 | __ | |aJohrat|c2022|bAssam Agricultural University, Johrat |
| 502 | __ | |bPhD|d2022|cAnimal Biotechnology, Assam Agricultural University, Johrat, Johrat |
| 518 | __ | |oDate of Award|d2022 |
| 518 | __ | |oDate of Registration|d2017 |
| 518 | __ | |oDate of Completion|d2022 |
| 520 | __ | |aClostridium perfringens causes several forms of enteric disease in human and animals. Different toxinotypes of C. perfringens produce different combinations of lethal toxins. C. perfringens type C produces beta toxin which is a kind of lethal pore-forming toxin that is responsible for necrotic enteritis in animals. Although C. perfringens type C infects several livestock species, the juvenile pigs are most susceptible. In India, the north-eastern states have the highest pig population in the country but no indigenous vaccine against C. perfringens type C is currently available. Therefore, the present study was conducted with an aim to produce recombinant beta toxin protein in a heterologous host and to evaluate the immunogenicity of the recombinant toxin adjuvanted with calcium phosphate nanoparticles in the mice model. For this, the cpb gene of C. perfringens type C that encodes the beta toxin was cloned into a prokaryotic expression vector, pET28a(+). Then the recombinant clone was transformed into BL21-C |
| 650 | __ | |aBiotechnology|2UGC |
| 650 | __ | |aLife Science|2AIU |
| 653 | __ | |aPlant and Animal Science |
| 700 | __ | |aSharma, R K|eGuide |
| 856 | __ | |uhttp://shodhganga.inflibnet.ac.in/handle/10603/472101|yShodhganga |
| 905 | __ | |afromsg |
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