| Type of Material: | Thesis |
| Title: | Isolation characterization and morpho biometric evaluation of pre pubertal porcine spermatogonial stem cells in different culture media |
| Researcher: | Lalmalsawma, Timothy |
| Guide: | Das, Arpana |
| Department: | Animal Genetics and Breeding |
| Publisher: | Assam Agricultural University, Johrat |
| Place: | Johrat |
| Year: | 2022 |
| Language: | English |
| Subject: | Animal Genetics | Biotechnology | Life Science |
| Dissertation/Thesis Note: | PhD; Animal Genetics and Breeding, Assam Agricultural University, Johrat, Johrat; 2022 |
| Fulltext: | Shodhganga |
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| 035 | __ | |a(IN-AhILN)th_462903 |
| 040 | __ | |dIN-AhILN|aASAM_785013 |
| 041 | __ | |aeng |
| 100 | __ | |eResearcher|aLalmalsawma, Timothy |
| 110 | __ | |aAnimal Genetics and Breeding|0U-0048|dJohrat|bAssam Agricultural University, Johrat|ein |
| 245 | __ | |aIsolation characterization and morpho biometric evaluation of pre pubertal porcine spermatogonial stem cells in different culture media |
| 260 | __ | |aJohrat|c2022|bAssam Agricultural University, Johrat |
| 502 | __ | |bPhD|d2022|cAnimal Genetics and Breeding, Assam Agricultural University, Johrat, Johrat |
| 518 | __ | |oDate of Award|d2022 |
| 518 | __ | |oDate of Registration|d2018 |
| 518 | __ | |oDate of Completion|d2022 |
| 520 | __ | |aTestes samples were collected from 7-15 days old pre-pubertal male crossbred piglets (Local × Hampshire) for isolation, enrichment and in vitro culture of porcine spermatogonial stem cells (SSCs). Isolation of spermatogonial stem cells like cells was performed by double enzymatic digestion using four enzymes viz., collagenase, DNase I, hyaluronidase type II and trypsin-EDTA. Isolated cells were further enriched by differential plating and percoll density gradient centrifugation method. Enriched cells were cultured on Sertoli cell feeder layer in three different culture media. All the three media consisted of same concentration of DMEM, NEEA, L-glutamine, FBS, EGF and FGF, however in addition to these, LIF was added to media I, GDNF was added to media II and both LIF and GDNF were added to media III. Characterization of SSCs was done by alkaline phosphatase and immunoflourescence staining. Expression of SSC specific pluripotent marker genes by putative SSCs was also studied by RT-PCR study. Porcine SSCs wer |
| 650 | __ | |aBiotechnology|2UGC |
| 650 | __ | |aLife Science|2AIU |
| 653 | __ | |aAnimal Genetics |
| 700 | __ | |aDas, Arpana|eGuide |
| 856 | __ | |uhttp://shodhganga.inflibnet.ac.in/handle/10603/472072|yShodhganga |
| 905 | __ | |afromsg |
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