| Type of Material: | Thesis |
| Title: | Studies of proteases from biological sources |
| Researcher: | Gaur, Smriti |
| Guide: | Wadhwa, Neeraj |
| Department: | Department of Biotechnology |
| Publisher: | Jaypee University of Information Technology, Solan |
| Place: | Waknaghat, PO. Teh. Kandaghat |
| Year: | 2010 |
| Language: | English |
| Subject: | Lantana camara | Pseudomonas thermaerum | Proteases | Classification of Proteases | Sources of Proteases | Microbiology | Life Science |
| Dissertation/Thesis Note: | PhD |
| Fulltext: | Shodhganga |
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| 001 | 459736 | |
| 003 | IN-AhILN | |
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| 008 | __ | 260508t2010||||ii#||||g|m||||||||||eng|| |
| 035 | __ | |a(IN-AhILN)th_459736 |
| 040 | __ | |dIN-AhILN|aJUIT_173215 |
| 041 | __ | |aeng |
| 100 | __ | |eResearcher|aGaur, Smriti |
| 110 | __ | |aDepartment of Biotechnology|0U-0186|dWaknaghat, PO. Teh. Kandaghat|bJaypee University of Information Technology, Solan|ein |
| 245 | __ | |aStudies of proteases from biological sources |
| 260 | __ | |aWaknaghat, PO. Teh. Kandaghat|c2010|bJaypee University of Information Technology, Solan |
| 502 | __ | |bPhD |
| 518 | __ | |d2010|oDate of Award |
| 518 | __ | |oDate of Completion|d2010 |
| 520 | __ | |aAn extracellular protease was purified from Pseudomonas thermaerum GW1 a new strain identified by morphological, biochemical and 16S rDNA sequencing. It was isolated from soil of Poultry waste site at Ghazipur near Ghaziabad, Delhi. This strain produces extra cellular protease in the culture media that was maintained at 37°C at 140 rpm after 48 hrs of incubation. Purification steps involved ammonium sulphate precipitation, DEAE-cellulose chromatography followed by casein zymography studies. Enzyme remained stable between at 60°C at pH 8.0. Interestingly Mn2+ strongly activated enzyme activity by 5 fold, where as Zn2+, Fe2+ and Hg2+ inhibited enzyme activity. The protease was stable in presence of 50 % (v/v) ethylacetate and acetone whereas isopropanol, methanol and benzene increased protease activity by 2.7, 1.3 and 1.1 fold respectively suggesting its potential industrial application. Additionally protease from senesced leaves of the weed Lantana camara was purified by a two-step procedure involving ammo |
| 650 | __ | |aMicrobiology|2UGC |
| 650 | __ | |aLife Science|2AIU |
| 653 | __ | |aLantana camara |
| 653 | __ | |aPseudomonas thermaerum |
| 653 | __ | |aProteases |
| 653 | __ | |aClassification of Proteases |
| 653 | __ | |aSources of Proteases |
| 700 | __ | |aWadhwa, Neeraj|eGuide |
| 856 | __ | |uhttp://shodhganga.inflibnet.ac.in/handle/10603/2407|yShodhganga |
| 905 | __ | |afromsg |
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