Title : Isolation Phenotypic and Genotypic Characterization of Non Tuberculous Mycobacteria isolated from Clinical Specimens

Type of Material: Thesis
Title: Isolation Phenotypic and Genotypic Characterization of Non Tuberculous Mycobacteria isolated from Clinical Specimens
Researcher: PRAVEEN KUMAR, V
Guide: SREENIVASULUREDDY, V
Department: Faculty of Medical Sciences
Publisher: Bharath University, Chennai
Place: Chennai
Year: 2020
Language: English
Subject: Life Sciences
Microbiology
Medical Sciences
Life Science
Dissertation/Thesis Note: PhD; Faculty of Medical Sciences, Bharath University, Chennai, Chennai; 2020; D14MS007
Fulltext: Shodhganga

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035__|a(IN-AhILN)th_454714
040__|aBHAU_600073|dIN-AhILN
041__|aeng
100__|aPRAVEEN KUMAR, V|eResearcher
110__|aFaculty of Medical Sciences|bBharath University, Chennai|dChennai|ein
245__|aIsolation Phenotypic and Genotypic Characterization of Non Tuberculous Mycobacteria isolated from Clinical Specimens
260__|aChennai|bBharath University, Chennai|c2020
300__|dDVD
502__|bPhD|cFaculty of Medical Sciences, Bharath University, Chennai, Chennai|d2020|oD14MS007
520__|aNon tuberculous mycobacteriais an opportunistic pathogen reported in the recent years among both immunodeficient and immunocompetent hosts. The aim of this study is to know the incidence of NTM species in pulmonary and extrapulmonary tuberculosis cases in a tertiary care hospital in Pondicherry and to evaluate different phenotypic and genotypic methods in identifying NTM and finally to determine their DST patterns. According to PCR-RFLP analysis of hsp65 gene, 253 (56.22%) were identified as M.abscessus followed by M.simiae 86 (19.11%), 58 (12.88%) as M.fortuitum, 49 (10.88%) M.chelonae and 4(0.88) were identified as M.gordonae. PCR RFLP of 16S-23S rRNA ITS gene results were contradictory, of the 452 isolates 2 isolates were identified as M.tb complex, ITS gene of M.abscessus has no restriction sites for both BstEII and HaeIII enzymes. The ITS gene of 86 isolates of M.simiae produced restriction fragments with HaeIII and the gene was not digested with BstEII enzyme.Hsp5 gene sequencing of the 450 NTM specie
650__|aMedical Sciences|2UGC
650__|aLife Science|2AIU
653__|aLife Sciences
653__|aMicrobiology
700__|aSREENIVASULUREDDY, V|eGuide
856__|uhttp://shodhganga.inflibnet.ac.in/handle/10603/311567|yShodhganga
905__|afromsg

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