Type of Material: | Thesis |
Title: | Isolation Phenotypic and Genotypic Characterization of Non Tuberculous Mycobacteria isolated from Clinical Specimens |
Researcher: | PRAVEEN KUMAR, V |
Guide: | SREENIVASULUREDDY, V |
Department: | Faculty of Medical Sciences |
Publisher: | Bharath University, Chennai |
Place: | Chennai |
Year: | 2020 |
Language: | English |
Subject: | Life Sciences | Microbiology | Medical Sciences | Life Science |
Dissertation/Thesis Note: | PhD; Faculty of Medical Sciences, Bharath University, Chennai, Chennai; 2020; D14MS007 |
Fulltext: | Shodhganga |
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035 | __ | |a(IN-AhILN)th_454714 |
040 | __ | |aBHAU_600073|dIN-AhILN |
041 | __ | |aeng |
100 | __ | |aPRAVEEN KUMAR, V|eResearcher |
110 | __ | |aFaculty of Medical Sciences|bBharath University, Chennai|dChennai|ein |
245 | __ | |aIsolation Phenotypic and Genotypic Characterization of Non Tuberculous Mycobacteria isolated from Clinical Specimens |
260 | __ | |aChennai|bBharath University, Chennai|c2020 |
300 | __ | |dDVD |
502 | __ | |bPhD|cFaculty of Medical Sciences, Bharath University, Chennai, Chennai|d2020|oD14MS007 |
520 | __ | |aNon tuberculous mycobacteriais an opportunistic pathogen reported in the recent years among both immunodeficient and immunocompetent hosts. The aim of this study is to know the incidence of NTM species in pulmonary and extrapulmonary tuberculosis cases in a tertiary care hospital in Pondicherry and to evaluate different phenotypic and genotypic methods in identifying NTM and finally to determine their DST patterns. According to PCR-RFLP analysis of hsp65 gene, 253 (56.22%) were identified as M.abscessus followed by M.simiae 86 (19.11%), 58 (12.88%) as M.fortuitum, 49 (10.88%) M.chelonae and 4(0.88) were identified as M.gordonae. PCR RFLP of 16S-23S rRNA ITS gene results were contradictory, of the 452 isolates 2 isolates were identified as M.tb complex, ITS gene of M.abscessus has no restriction sites for both BstEII and HaeIII enzymes. The ITS gene of 86 isolates of M.simiae produced restriction fragments with HaeIII and the gene was not digested with BstEII enzyme.Hsp5 gene sequencing of the 450 NTM specie |
650 | __ | |aMedical Sciences|2UGC |
650 | __ | |aLife Science|2AIU |
653 | __ | |aLife Sciences |
653 | __ | |aMicrobiology |
700 | __ | |aSREENIVASULUREDDY, V|eGuide |
856 | __ | |uhttp://shodhganga.inflibnet.ac.in/handle/10603/311567|yShodhganga |
905 | __ | |afromsg |
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