Title : PROCESS FACILITATED ENHANCEMENT OF PRODUCTION AND PURIFICATION OF LIPASE USING BACILLUS SPP

Type of Material: Thesis
Title: PROCESS FACILITATED ENHANCEMENT OF PRODUCTION AND PURIFICATION OF LIPASE USING BACILLUS SPP
Researcher: M. SATHISH KUMAR
Guide: C.M.Karrunakaran
Department: Department of Engineering and Technology(Industrial Biotechnology)
Publisher: Bharath University, Chennai
Place: Chennai
Year: 2013
Language: English
Subject: Biotechnology
Engineering and Technology
Dissertation/Thesis Note: PhD; Department of Engineering and Technology(Industrial Biotechnology), Bharath University, Chennai, Chennai; 2013; D05BT015
Fulltext: Shodhganga

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035__|a(IN-AhILN)th_454559
040__|aBHAU_600073|dIN-AhILN
041__|aeng
100__|aM. SATHISH KUMAR|eResearcher
110__|aDepartment of Engineering and Technology(Industrial Biotechnology)|bBharath University, Chennai|dChennai|ein
245__|aPROCESS FACILITATED ENHANCEMENT OF PRODUCTION AND PURIFICATION OF LIPASE USING BACILLUS SPP
260__|aChennai|bBharath University, Chennai|c2013
300__|dDVD
502__|bPhD|cDepartment of Engineering and Technology(Industrial Biotechnology), Bharath University, Chennai, Chennai|d2013|oD05BT015
518__|oDate of Registration|d2005-01-26
520__|aLipase is the extensively used key enzyme for various biotechnological applications in wide spectrum of industries. The process oriented feeding strategies for higher production of lipase was objective of the study. Range of carbon sources were used for the higher production of lipase, among them germinated maize oil was chosen as the best one. Feeding strategies like quasi steady state, pH stat and DO stat were employed. Eventually it was established that the DO stat based fed batch fermentation gave maximum in terms of both enzymatic units and productivity. The yield in DO stat fed batch fermentation was seven fold higher when compared to batch fermentation. The optimum temperature and pH was arrived at from a range of temperature and pH. Further the activity of lipase was observed by using different pH and temperatures. It was found that at pH 6.5 and temperature at 37oC the lipase enzyme was most active. The purification of the lipase done by ammonium sulphate precipitation and two stage chromatographic
650__|aBiotechnology|2UGC
650__|aEngineering and Technology|2AIU
700__|aC.M.Karrunakaran|eGuide
856__|uhttp://shodhganga.inflibnet.ac.in/handle/10603/170909|yShodhganga
905__|afromsg

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