Type of Material: | Thesis |
Title: | PROCESS FACILITATED ENHANCEMENT OF PRODUCTION AND PURIFICATION OF LIPASE USING BACILLUS SPP |
Researcher: | M. SATHISH KUMAR |
Guide: | C.M.Karrunakaran |
Department: | Department of Engineering and Technology(Industrial Biotechnology) |
Publisher: | Bharath University, Chennai |
Place: | Chennai |
Year: | 2013 |
Language: | English |
Subject: | Biotechnology | Engineering and Technology |
Dissertation/Thesis Note: | PhD; Department of Engineering and Technology(Industrial Biotechnology), Bharath University, Chennai, Chennai; 2013; D05BT015 |
Fulltext: | Shodhganga |
000 | 00000ntm a2200000ua 4500 | |
001 | 454559 | |
003 | IN-AhILN | |
005 | 2024-09-13 17:51:28 | |
008 | __ | 240913t2013||||ii#||||g|m||||||||||eng|| |
035 | __ | |a(IN-AhILN)th_454559 |
040 | __ | |aBHAU_600073|dIN-AhILN |
041 | __ | |aeng |
100 | __ | |aM. SATHISH KUMAR|eResearcher |
110 | __ | |aDepartment of Engineering and Technology(Industrial Biotechnology)|bBharath University, Chennai|dChennai|ein |
245 | __ | |aPROCESS FACILITATED ENHANCEMENT OF PRODUCTION AND PURIFICATION OF LIPASE USING BACILLUS SPP |
260 | __ | |aChennai|bBharath University, Chennai|c2013 |
300 | __ | |dDVD |
502 | __ | |bPhD|cDepartment of Engineering and Technology(Industrial Biotechnology), Bharath University, Chennai, Chennai|d2013|oD05BT015 |
518 | __ | |oDate of Registration|d2005-01-26 |
520 | __ | |aLipase is the extensively used key enzyme for various biotechnological applications in wide spectrum of industries. The process oriented feeding strategies for higher production of lipase was objective of the study. Range of carbon sources were used for the higher production of lipase, among them germinated maize oil was chosen as the best one. Feeding strategies like quasi steady state, pH stat and DO stat were employed. Eventually it was established that the DO stat based fed batch fermentation gave maximum in terms of both enzymatic units and productivity. The yield in DO stat fed batch fermentation was seven fold higher when compared to batch fermentation. The optimum temperature and pH was arrived at from a range of temperature and pH. Further the activity of lipase was observed by using different pH and temperatures. It was found that at pH 6.5 and temperature at 37oC the lipase enzyme was most active. The purification of the lipase done by ammonium sulphate precipitation and two stage chromatographic |
650 | __ | |aBiotechnology|2UGC |
650 | __ | |aEngineering and Technology|2AIU |
700 | __ | |aC.M.Karrunakaran|eGuide |
856 | __ | |uhttp://shodhganga.inflibnet.ac.in/handle/10603/170909|yShodhganga |
905 | __ | |afromsg |
User Feedback Comes Under This section.