Type of Material: | Thesis |
Title: | Reproductive biology and tissue culturestudies in "Winged Bean" [Psophocarpus tetragonolobus (L.)DC] |
Researcher: | Koshy, Eapen P |
Guide: | John, Philip |
Department: | Faculty of Science |
Publisher: | Mahatma Gandhi University, Kottayam |
Place: | Kottayam |
Year: | 2007/03 |
Language: | English |
Subject: | Biochemical analysis | Winged bean | Psophocarpus tetragonolobus | Tissue culture | Thin cell layer explants | Left cotyledon | Right cotyledon | Reproductive biology | RAPD | Protein profile | Botany | Life Science |
Dissertation/Thesis Note: | PhD; 2007/03 |
Fulltext: | Shodhganga |
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008 | __ | 240911t1970||||ii#||||g|m||||||||||eng|| |
035 | __ | |a(IN-AhILN)th_454433 |
040 | __ | |aMGND_686560|dIN-AhILN |
041 | __ | |aeng |
100 | __ | |aKoshy, Eapen P|eResearcher |
110 | __ | |aFaculty of Science|bMahatma Gandhi University, Kottayam|dKottayam|ein |
245 | __ | |aReproductive biology and tissue culturestudies in "Winged Bean" [Psophocarpus tetragonolobus (L.)DC] |
260 | __ | |aKottayam|bMahatma Gandhi University, Kottayam|c2007/03 |
300 | __ | |a134p.|c-|dNone |
500 | __ | |aBibliography includes |
502 | __ | |bPhD|d2007/03 |
518 | __ | |dn.d.|oDate of Award |
518 | __ | |dn.d.|oDate of Registration |
520 | __ | |aPsophocarpus tetragonolobus (L.) DC., a potential backyard crop, has immense agricultural possibilities. The present study describes the floral biology of the plant and proposes successful micropropagation protocols. A detailed description of the plant was prepared along with a diagrammatic sketch. The morphology and ultrastructure of the reproductive organs were studied. Cytochemical analysis of the stigma reveals the localization of starch, lipids and proteins at various developmental stages. Thin cell layer nodal and stem explants gave maximum shoot formation, while whole tissue explants were inferior. The right cotyledon was superior to the left cotyledon in shoot regeneration. A three stage culture regime was necessary for successful callus regeneration induction, maintenance and regeneration stages. BAP + GA3 medium induced shoot elongation. Differentiated shoots were rooted to get healthy plantlets. Somatic embryos were initiated from leaf explants when 2,4-D induced callus was transferred to TDZ c |
650 | __ | |aBotany|2UGC |
650 | __ | |aLife Science|2AIU |
653 | __ | |aBiochemical analysis |
653 | __ | |aWinged bean |
653 | __ | |aPsophocarpus tetragonolobus |
653 | __ | |aTissue culture |
653 | __ | |aThin cell layer explants |
653 | __ | |aLeft cotyledon |
653 | __ | |aRight cotyledon |
653 | __ | |aReproductive biology |
653 | __ | |aRAPD |
653 | __ | |aProtein profile |
700 | __ | |aJohn, Philip|eGuide |
856 | __ | |uhttp://shodhganga.inflibnet.ac.in/handle/10603/7107|yShodhganga |
905 | __ | |afromsg |
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