Title : Reproductive biology and tissue culturestudies in "Winged Bean" [Psophocarpus tetragonolobus (L.)DC]

Type of Material: Thesis
Title: Reproductive biology and tissue culturestudies in "Winged Bean" [Psophocarpus tetragonolobus (L.)DC]
Researcher: Koshy, Eapen P
Guide: John, Philip
Department: Faculty of Science
Publisher: Mahatma Gandhi University, Kottayam
Place: Kottayam
Year: 2007/03
Language: English
Subject: Biochemical analysis
Winged bean
Psophocarpus tetragonolobus
Tissue culture
Thin cell layer explants
Left cotyledon
Right cotyledon
Reproductive biology
RAPD
Protein profile
Botany
Life Science
Dissertation/Thesis Note: PhD; 2007/03
Fulltext: Shodhganga

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035__|a(IN-AhILN)th_454433
040__|aMGND_686560|dIN-AhILN
041__|aeng
100__|aKoshy, Eapen P|eResearcher
110__|aFaculty of Science|bMahatma Gandhi University, Kottayam|dKottayam|ein
245__|aReproductive biology and tissue culturestudies in "Winged Bean" [Psophocarpus tetragonolobus (L.)DC]
260__|aKottayam|bMahatma Gandhi University, Kottayam|c2007/03
300__|a134p.|c-|dNone
500__|aBibliography includes
502__|bPhD|d2007/03
518__|dn.d.|oDate of Award
518__|dn.d.|oDate of Registration
520__|aPsophocarpus tetragonolobus (L.) DC., a potential backyard crop, has immense agricultural possibilities. The present study describes the floral biology of the plant and proposes successful micropropagation protocols. A detailed description of the plant was prepared along with a diagrammatic sketch. The morphology and ultrastructure of the reproductive organs were studied. Cytochemical analysis of the stigma reveals the localization of starch, lipids and proteins at various developmental stages. Thin cell layer nodal and stem explants gave maximum shoot formation, while whole tissue explants were inferior. The right cotyledon was superior to the left cotyledon in shoot regeneration. A three stage culture regime was necessary for successful callus regeneration induction, maintenance and regeneration stages. BAP + GA3 medium induced shoot elongation. Differentiated shoots were rooted to get healthy plantlets. Somatic embryos were initiated from leaf explants when 2,4-D induced callus was transferred to TDZ c
650__|aBotany|2UGC
650__|aLife Science|2AIU
653__|aBiochemical analysis
653__|aWinged bean
653__|aPsophocarpus tetragonolobus
653__|aTissue culture
653__|aThin cell layer explants
653__|aLeft cotyledon
653__|aRight cotyledon
653__|aReproductive biology
653__|aRAPD
653__|aProtein profile
700__|aJohn, Philip|eGuide
856__|uhttp://shodhganga.inflibnet.ac.in/handle/10603/7107|yShodhganga
905__|afromsg

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