| Type of Material: | Thesis |
| Title: | Phosphatidyl Inositol 3 kinases inhibitor development using novel invitro pharmacological assays |
| Researcher: | Mahesh Yanamandra |
| Guide: | Sayan Mitra | Archana Giri |
| Department: | Faculty of Biotechnology |
| Publisher: | Jawaharlal Nehru Technological University, Hyderabad |
| Place: | Hyderabad |
| Year: | 2015 |
| Language: | English |
| Subject: | Biotechnology and Applied Microbiology | Life Sciences | Microbiology | Biotechnology | Engineering and Technology |
| Dissertation/Thesis Note: | PhD; Faculty of Biotechnology, Jawaharlal Nehru Technological University, Hyderabad, Hyderabad; 2015 |
| Fulltext: | Shodhganga |
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| 001 | 454232 | |
| 003 | IN-AhILN | |
| 005 | 2024-07-29 17:30:51 | |
| 008 | __ | 240729t2015||||ii#||||g|m||||||||||eng|| |
| 035 | __ | |a(IN-AhILN)th_454232 |
| 040 | __ | |aJNTU_500028|dIN-AhILN |
| 041 | __ | |aeng |
| 100 | __ | |aMahesh Yanamandra|eResearcher |
| 110 | __ | |aFaculty of Biotechnology|bJawaharlal Nehru Technological University, Hyderabad|dHyderabad|ein|0U-0017 |
| 245 | __ | |aPhosphatidyl Inositol 3 kinases inhibitor development using novel invitro pharmacological assays |
| 260 | __ | |aHyderabad|bJawaharlal Nehru Technological University, Hyderabad|c2015 |
| 300 | __ | |a197p.|dDVD |
| 502 | __ | |cFaculty of Biotechnology, Jawaharlal Nehru Technological University, Hyderabad, Hyderabad|d2015|bPhD |
| 518 | __ | |dSeptember 2015|oDate of Award |
| 518 | __ | |oDate of Registration|d2010-01-01 |
| 520 | __ | |aThe phosphatidyl inositol 3-kinases(PI3Ks) are lipid kinases that regulate the newlinecellular signal transduction pathways involved in cell growth, proliferation, newlinesurvival, apoptosis and adhesion. Deregulations of these pathways are common newlinein oncogenesis and are known to be altered in other metabolic disorders as well. In spite of its huge potential as an attractive target in the above diseases, newlinethere is an unmet need for the development of a successful inhibitor. Unlike protein kinase inhibitor, screening for lipid kinase inhibitor has been challenging due to the lipidic nature of PI3K substrates known as phosphoinositides. Here we report for the first time, the development of unique and novel radioactive lipid kinase screening platform using phosphocellulose newlinepaper that involves transfer of adiolabelled [?P32] ATP to phosphatidylinositol 4,5 phosphate forming Phosphatidylinositol-3,4,5 phosphate captured on the phosphocellulose matrix. Enzyme kinetics and inhibitory properties |
| 650 | __ | |aBiotechnology|2UGC |
| 650 | __ | |aEngineering and Technology|2AIU |
| 653 | __ | |aBiotechnology and Applied Microbiology |
| 653 | __ | |aLife Sciences |
| 653 | __ | |aMicrobiology |
| 700 | __ | |aSayan Mitra|eGuide |
| 700 | __ | |eCo-Guide|aArchana Giri |
| 856 | __ | |uhttp://shodhganga.inflibnet.ac.in/handle/10603/291073|yShodhganga |
| 905 | __ | |afromsg |
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