| Type of Material: | Thesis |
| Title: | Functional Validation of Novel Rice gene for its role in flower and Seed development through over expression and silencing approach |
| Researcher: | Manimaran, P. |
| Guide: | Balachandran, S. M. |
| Department: | Faculty of Biotechnology |
| Publisher: | Jawaharlal Nehru Technological University, Hyderabad |
| Place: | Hyderabad |
| Year: | 2013 |
| Language: | English |
| Subject: | Functional | Novel Rice | silencing | Validation | Biotechnology | Engineering and Technology |
| Dissertation/Thesis Note: | PhD; Faculty of Biotechnology, Jawaharlal Nehru Technological University, Hyderabad, Hyderabad; 2013 |
| Fulltext: | Shodhganga |
| 000 | 00000ntm a2200000ua 4500 | |
| 001 | 453894 | |
| 003 | IN-AhILN | |
| 005 | 2024-07-04 17:52:26 | |
| 008 | __ | 240704t2013||||ii#||||g|m||||||||||eng|| |
| 035 | __ | |a(IN-AhILN)th_453894 |
| 040 | __ | |aJNTU_500028|dIN-AhILN |
| 041 | __ | |aeng |
| 100 | __ | |aManimaran, P.|eResearcher |
| 110 | __ | |aFaculty of Biotechnology|bJawaharlal Nehru Technological University, Hyderabad|dHyderabad|ein|0U-0017 |
| 245 | __ | |aFunctional Validation of Novel Rice gene for its role in flower and Seed development through over expression and silencing approach |
| 260 | __ | |aHyderabad|bJawaharlal Nehru Technological University, Hyderabad|c2013 |
| 300 | __ | |a179 p.|dNone|c- |
| 500 | __ | |aReferences p. 145-179 , Appendix p i-xiii |
| 502 | __ | |cFaculty of Biotechnology, Jawaharlal Nehru Technological University, Hyderabad, Hyderabad|d2013|bPhD |
| 520 | __ | |aCalcium dependent protein kinases (CDPKs) are Ca2+ sensor protein kinases that are prevalent in plants. They play diverse roles in plant signaling mechanism. Thirty one CDPK genes have been identified in rice, of which a few are involved during seed development newlineprocess and some are functionally haracterized. Expression profiling of this gene in indica genotype indicated that the gene expressed only in endosperm. The present work was undertaken to validate the newly newlineidentified CDPK gene, OsCPK31 through transgenic approach in BPT 5204 and Taipei 309 rice genotypes. Putative transgenic plants were developed in both genotypes with two gene constructs viz., overexpression and silencing vectors and they were confirmed by PCR and Southern analysis for transgene integration. Selected transgenic newlineplants were advanced up to T3 generation and all the transgenic plants were confirmed by PCR and Southern hybridization. Northern newlinedot-blot analysis of non-transformed control plant (BPT 5204) rev |
| 650 | __ | |aBiotechnology|2UGC |
| 650 | __ | |aEngineering and Technology|2AIU |
| 653 | __ | |aFunctional |
| 653 | __ | |aNovel Rice |
| 653 | __ | |asilencing |
| 653 | __ | |aValidation |
| 700 | __ | |aBalachandran, S. M.|eGuide |
| 856 | __ | |uhttp://shodhganga.inflibnet.ac.in/handle/10603/19264|yShodhganga |
| 905 | __ | |afromsg |
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